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Autor: Alvarado Tapias, E.A ; Rivas Coppola, M.S ; Vásquez Pérez, A.V ; Torres Peraza, J. F ; Vílchez Pineda, D ; Bravo Toba, I. D ; Bonfante Cabarcas, Rafael Armando ; Rivas Coppola, M.S ; Vásquez Pérez, A.V ; Torres Peraza, J. F ; Vílchez Pineda, D ; Bravo Toba, I. D ; Bonfante Cabarcas, Rafael Armando
Título: EFECTO DE LA HEPARINA, EL MAGNESIO Y LA VITAMINA E SOBRE LA NEUROTOXICIDAD INDUCIDA POR ISQUEMIA EN RATAS SPRAGUE-DAWLEY
HEPARIN, MAGNESIUM AND VITAMIN E EFFECT ON NEUROTOXICITY INDUCE BY ISCHEMIC IN SPRAGUE DAWLEY RATS
ISSN: 0798-0361
Fecha: Octubre - Diciembre 2014
Páginas/Colación: p. 262-272
En:/ BOLETÍN MÉDICO DE POSTGRADO Vol. XXX Octubre - Diciembre 2014
Información de existenciaInformación de existencia
Categoría Temática: Palabras: MED01 MED01
Palabras Claves del Autor: Palabras: HEPARINA HEPARINA, Palabras: ISQUEMIA ISQUEMIA, Palabras: MAGNESIO MAGNESIO, Palabras: MOTILIDAD MOTILIDAD, Palabras: NEUROTOXICIDAD NEUROTOXICIDAD, Palabras: VITAMINA E VITAMINA E
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RESUMEN
La morbimortalidad por accidentes cerebro vasculares representan un problema de salud pública en Venezuela. Se ha postulado que durante los períodos de hipoxia: la producción de radicales libres, la entrada masiva de calcio, la activación de los receptores NMDA y la producción de un fragmento neurotóxico de 22 kDa a través de la acción de la trombina sobre la apolipoproteína E están involucrados en la neurotoxicidad. En el presente trabajo evaluamos clínicamente el efecto del Mg2+, la heparina, las vitaminas E y C sobre la morbi-mortalidad en ratas Sprague Dwaley sometidas a isquemia cerebral global por ligadura y corte de ambas arterias carótidas. La muestra estuvo constituida por 40 animales, divididas en 5 grupos: Control-Control, Control-Fisiológica, magnesio, vitamina E y heparina, aplicando un protocolo doble ciego. Bajo anestesia general los animales fueron sometidos a ligadura, corte, canulación e infusión de los fármacos experimentales en la carótida común izquierda, y ligadura y corte de la carótida común derecha. Por las cánulas se administró solución fisiológica, MgCl2 en solución fisiológica y heparina. Las vitaminas E y vitamina C se administraron vía oral 6 días en el pre-operatoria. Se realizaron evaluaciones motoras y conductuales pre-operatorias y post-operatorias. Se observó la más alta mortalidad en el grupo Control-Control y el menor porcentaje fue observado en el grupo Control-Fisiológica y heparina. El grupo heparina presentó el mayor promedio de motilidad y el mayor promedio de conducta exploratoria. El grupo magnesio presentó el menor promedio de ptosis palpebral y el menor promedio de trastornos motores. A su vez el grupo vitamina E presentó el mayor período de sobrevida post-operatoria. En conclusión la heparina, el magnesio y la vitamina E previenen la neurotoxicidad disminuyendo la mortalidad y la morbilidad.
PALABRAS CLAVEs DEL AUTOR: Heparina. Magnesio. Vitamina E. Neurotoxicidad. Isquemia. Motilidad.

ABSTRACT
HEPARIN, MAGNESIUM AND VITAMIN E EFFECT ON NEUROTOXICITY INDUCE BY ISCHEMIC IN SPRAGUE DAWLEY RATS
The morbidity and mortality from strokes represent a public health problem in Venezuela. It is postulated that during periods of hypoxia: the production of free radicals, massive calcium influx, activation of the NMDA receptors and the production of 22 kDa neurotoxic fragment from the action of thrombin on apolipoprotein E are involved in the neurotoxicity. In this study we clinically evaluated the effect of Mg2+, heparin, vitamins E and C on morbidity and mortality of adult male Sprague Dawley subjected to global cerebral ischemia by ligation and cut of both carotid arteries. The sample consisted of 40 animals, divided into 5 groups: Control-Control, Control-Vehicle, magnesium, vitamin E/C and heparin, using a double-blind protocol. Under general anesthesia the animals underwent ligation, cut, cannulation and infusion of experimental drugs into the left common carotid artery, and ligation and cut of the right common carotid. Through the cannulas were administered saline, MgCl2 saline and heparin. Vitamins E and vitamin C were administered orally 6 days in the pre -operative period. Pre-operative and post-operative motor and behavioral assessments were performed. The highest mortality was observed in the Control-Control group and the lowest percentage was observed in the Control-Vehicle and heparin groups. The heparin group had the highest average in motility and in rearing behavior. The magnesium group had the lowest average in ptosis and motor disorders. In turn, the vitamin E/C the highest post-surgical survival. In conclusion heparin, magnesium and vitamin E prevent neurotoxicity decreasing mortality and/or morbidity.
KEY WORDS: Heparin. Magnesium. Vitamin E. Neurotoxicity. Ischemia. Motility.

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